Mallmann P, Schröder W, van der Ven H, Diedrich K, Krebs K
Department of Gynecology and Obstetrics, Bonn, Germany.
Andrologia. 1991 Jan-Feb;23(1):41-3. doi: 10.1111/j.1439-0272.1991.tb02492.x.
In 13 healthy women and 6 virgins the cellular sensitization against sperm and seminal plasma antigens was demonstrated by an indirect lymphokin assay, the leucocyte migration inhibition test (LMI-test) using the following preparations: "washed" spermatozoa, seminal plasma and spermatozoa of the supernatant prepared with the "swim-up" technique. In both groups of women a cellular sensitization against sperm and seminal plasma antigens could be observed. Further, a dose dependent correlation was found in that way, that increasing concentrations of spermatozoa lead to an increased inhibition of macrophage migration. In virgins cellular sensitization against seminal plasma proteins did not differ from non-virgins, only the percentage of significant reactions in the LMI-Test was reduced. As low sperm concentrations (1 million ml-1), which represent best the physiological situation in the uterus, induced an enhanced migrations of macrophages the enhancement of macrophage migration is considered as physiological cellular sensitization of females against sperm-associated antigens.
在13名健康女性和6名处女中，通过间接淋巴因子测定法（即白细胞迁移抑制试验，LMI试验），使用以下制剂证明了对精子和精浆抗原的细胞致敏作用：“洗涤过的”精子、精浆以及用“上游”技术制备的上清液中的精子。在两组女性中均观察到了对精子和精浆抗原的细胞致敏作用。此外，还发现了一种剂量依赖性相关性，即精子浓度增加会导致巨噬细胞迁移抑制增加。在处女中，对精浆蛋白的细胞致敏作用与非处女无异，只是LMI试验中显著反应的百分比有所降低。由于低精子浓度（100万/ml）最能代表子宫内的生理状况，其可诱导巨噬细胞迁移增强，因此巨噬细胞迁移增强被认为是女性对精子相关抗原的生理性细胞致敏作用。
