Bonavida B, Braquet M, Lebow L T, Jewett A
Department of Microbiology and Immunology, UCLA School of Medicine, University of California at Los Angeles 90024.
J Lipid Mediat Cell Signal. 1994 Feb;9(1):19-25.
The heterogeneous nature of cytotoxic effector cells presents a difficult obstacle to the study of developmental, biochemical and molecular events governing cytotoxic T lymphocytes (CTL) and natural killer (NK) cell biology. In previous studies, there were no available methods, other than the single cell assay or micromanipulation techniques that could distinguish between lytic and non-lytic cells present in purified populations or clones. Further, there were no available means to isolate these subsets for further investigation. The studies presented here describe a novel approach to identify and isolate cells based on their functional characteristics. Thus, non-lytic free and conjugate forming cells as well as killer cells could be identified, quantified, and further purified by flow cytometric cell sorting for further investigations. Studies of several properties of isolated NK subpopulations (free, non-lytic binders, and killers) are presented.
细胞毒性效应细胞的异质性给研究调控细胞毒性T淋巴细胞（CTL）和自然杀伤（NK）细胞生物学的发育、生化及分子事件带来了艰巨的障碍。在以往的研究中，除了单细胞检测或显微操作技术外，没有其他可用方法能够区分纯化群体或克隆中存在的裂解细胞和非裂解细胞。此外，也没有可用的方法来分离这些亚群以进行进一步研究。本文介绍的研究描述了一种基于细胞功能特性来识别和分离细胞的新方法。因此，非裂解性游离细胞、结合形成细胞以及杀伤细胞可以通过流式细胞术细胞分选进行识别、定量，并进一步纯化以进行深入研究。文中还展示了对分离出的NK亚群（游离细胞、非裂解性结合细胞和杀伤细胞）的多种特性的研究。
