Kolb J P, Abadie A, Proschnicka-Chalufour A, de Gramont A, Poggioli J
U365 INSERM, Institut Curie, Paris, France.
J Lipid Mediat Cell Signal. 1994 Feb;9(1):27-35.
Signal transduction through ligation of the CD23 (Fc epsilon RII) molecule was analysed in human B cells and monocytes. Monoclonal antibodies directed against the IgE binding site of CD23 were found to trigger phosphoinositide hydrolysis and calcium mobilization in B cells, but not in monocytes. These early events were also obtained with an IgE+anti-IgE complex, which supposedly mimicks the physiological situation of a multivalent antigen inducing the cross-linking of cell-bound IgE. Redistribution of CD23 was also found to evoke cAMP accumulation both in B lymphocytes and monocytes. Moreover, we present evidence indicating a possible cross-talk between the IL-4- and CD23-induced second messengers. We suggest that the alternative transduction pathways elicited by ligation of CD23 are linked to the CD23 isoform(s) expressed by these cells and may result from their association with different sets of molecules.
在人B细胞和单核细胞中分析了通过CD23（FcεRII）分子连接的信号转导。发现针对CD23的IgE结合位点的单克隆抗体可触发B细胞中的磷酸肌醇水解和钙动员，但不能触发单核细胞中的此类反应。这些早期事件也可通过IgE +抗IgE复合物获得，该复合物推测模拟了诱导细胞结合IgE交联的多价抗原的生理情况。还发现CD23的重新分布可引起B淋巴细胞和单核细胞中的cAMP积累。此外，我们提供的证据表明IL-4和CD23诱导的第二信使之间可能存在相互作用。我们认为，CD23连接引发的替代转导途径与这些细胞表达的CD23同种型有关，并且可能是由于它们与不同分子组的关联所致。
