Tunney M M, Bonner M C, Keane P F, Gorman S P
School of Pharmacy, Queen's University of Belfast, UK.
Biomaterials. 1996 May;17(10):1025-9. doi: 10.1016/0142-9612(96)84679-5.
A need exists for ureteral stent materials capable of preventing or reducing encrustation. The aim of this study, therefore, was to develop an in vitro model producing biomaterial encrustation similar to that on stents in vivo. Three models were designed and evaluated. Polyurethane stent sections were immersed in human urine (37 degrees C, 5% CO2): (1) with and (2) without crushed human kidney stone and (3) in an artificial urine (37 degrees C, 5% CO2). Encrustation of similar composition, as determined by infrared spectroscopy, X-ray diffraction and energy dispersive X-ray analysis, formed on stent materials in vivo, in artificial urine and in human urine with crushed kidney stone. Magnesium ammonium phosphate (struvite) and calcium phosphate (hydroxyapatite) predominated in all encrustations. The reproducibility and ease of use of the artificial urine model provided optimum encrustation assessment of materials presently used in ureteral stents and evaluation of novel biomaterials.
需要能够预防或减少结垢的输尿管支架材料。因此，本研究的目的是建立一种体外模型，该模型产生的生物材料结垢与体内支架上的结垢相似。设计并评估了三种模型。将聚氨酯支架切片浸入人尿液中（37℃，5%二氧化碳）：（1）加入碾碎的人肾结石和（2）不加入碾碎的人肾结石，以及（3）浸入人工尿液中（37℃，5%二氧化碳）。通过红外光谱、X射线衍射和能量色散X射线分析确定，在体内、人工尿液和含有碾碎肾结石的人尿液中的支架材料上形成了成分相似的结垢。磷酸镁铵（鸟粪石）和磷酸钙（羟基磷灰石）在所有结垢中占主导地位。人工尿液模型的可重复性和易用性为目前用于输尿管支架的材料提供了最佳的结垢评估，并可用于新型生物材料的评估。
