Kurokawa K, Jibiki I, Matsuda H, Fukushima T, Tsuji S, Yamaguchi N, Hisada K
Department of Neuropsychiatry, Kanazawa University School of Medicine, Japan.
Brain Res. 1994 Apr 11;642(1-2):303-10. doi: 10.1016/0006-8993(94)90935-0.
To clarify experimentally whether benzodiazepine (Bz) receptor or regional cerebral blood flow (rCBF) imaging is more sensitive in the detection of epileptic foci, we simultaneously examined the Bz receptor and rCBF distribution changes in hippocampal kindled rabbits with in vivo double tracer autoradiography using 125I-labeled Ro 16-0154 (125I-Iomazenil) and 99mTc-labeled hexamethylpropylene amine oxime (99mTc-HMPAO). In visual and quantitative analyses, 125I-Iomazenil accumulation in brain slices extracted after the completion of the kindling was markedly and extensively decreased in the kindled right CA1 region, and further in the right temporal lobe, dentate gyrus, CA2, CA4, and bilateral CA3 regions, regarded as the propagated sites of seizure discharges. 99mTc-HMPAO accumulation was much more slightly and less extensively decreased in the right CA1, frontal, temporal and dentate gyri. Further, this decrease in 125I-Iomazenil accumulation was not due to neuropathological abnormalities, which consisted only of tissue damage corresponding to electrode track in the CA1. Both the kindled and propagated sites are known to have a possibility of acquiring epileptogenesis as experimental epileptic foci. These results suggest that Bz receptor imaging is much more sensitive in the detection of epileptic foci than rCBF imaging, and therefore that Bz receptor imaging is useful in clinical epilepsy.
为了通过实验明确苯二氮䓬（Bz）受体显像或局部脑血流（rCBF）显像在癫痫病灶检测中哪一个更敏感，我们使用125I标记的Ro 16-0154（125I-艾司西酞普兰）和99mTc标记的六甲丙二胺肟（99mTc-HMPAO），通过体内双示踪剂放射自显影术同时检测了海马点燃兔的Bz受体和rCBF分布变化。在视觉和定量分析中，点燃完成后提取的脑片中，125I-艾司西酞普兰在右侧点燃的CA1区以及右侧颞叶、齿状回、CA2、CA4和双侧CA3区（被视为癫痫放电的传播部位）的积聚明显且广泛减少。99mTc-HMPAO在右侧CA1、额叶、颞叶和齿状回的积聚减少更为轻微且范围更小。此外，125I-艾司西酞普兰积聚的减少并非由于神经病理学异常，后者仅包括CA1区与电极轨迹相对应的组织损伤。已知点燃部位和传播部位都有可能作为实验性癫痫病灶而发生癫痫形成。这些结果表明，Bz受体显像在癫痫病灶检测中比rCBF显像敏感得多，因此Bz受体显像在临床癫痫中有用。
