Jibiki I, Kurokawa K, Matsuda H, Fukushima T, Tsuji S, Yamaguchi N, Hisada K
Department of Neuropsychiatry, Kanazawa University School of Medicine, Ishikawa Prefecture, Japan.
Pharmacol Biochem Behav. 1994 Jun;48(2):553-6. doi: 10.1016/0091-3057(94)90570-3.
To clarify experimentally which of benzodiazepine (BZ) receptor or regional cerebral blood flow (rCBF) imaging is more sensitive in the detection of epileptic foci, we simultaneously examined the BZ receptor and rCBF distribution changes in hippocampal kindled rabbits with in vivo double tracer autoradiography using 125I-labeled Ro 16-0154 (125I-Iomazenil) and 99mTc-labeled hexamethyl-propyleneamine oxime (99mTc-HMPAO). In visual analysis of brain slices extracted after the intravenous injection of the double tracer following the kindling completion, 125I-Iomazenil accumulation was more markedly and extensively decreased in the kindled right CA1 region mimicking a primary epileptic focus than 99mTc-HMPAO accumulation. Further, this decrease in 125I-Iomazenil accumulation was not due to neuropathological abnormalities which consisted only of tissue damage corresponding to electrode track in the right CA1. These results suggest that BZ receptor imaging is more sensitive in the detection of epileptic foci than rCBF imaging and, therefore, that BZ receptor imaging is useful in clinical epilepsy.
为了通过实验明确苯二氮䓬（BZ）受体成像或局部脑血流（rCBF）成像在癫痫病灶检测中哪一种更敏感，我们使用125I标记的Ro 16 - 0154（125I - 碘西泮）和99mTc标记的六甲丙二胺肟（99mTc - HMPAO），通过体内双示踪剂放射自显影术同时检测海马点燃兔的BZ受体和rCBF分布变化。在点燃完成后静脉注射双示踪剂后提取脑切片的视觉分析中，与99mTc - HMPAO聚集相比，模拟原发性癫痫病灶的点燃右侧CA1区125I - 碘西泮聚集更明显且广泛地减少。此外，125I - 碘西泮聚集的这种减少并非由于神经病理异常，神经病理异常仅包括右侧CA1区与电极轨迹对应的组织损伤。这些结果表明，BZ受体成像在癫痫病灶检测中比rCBF成像更敏感，因此，BZ受体成像在临床癫痫中有用。
