Lahm H, Wyniger J, Hertig S, Yilmaz A, Fischer J R, Givel J C, Odartchenko N
Department of Cellular Biology, Swiss Institute for Experimental Cancer Research, Epalinges.
Cancer Res. 1994 Jul 15;54(14):3700-2.
Secretion of several cytokines by colorectal carcinoma cells has been substantiated. These do not include granulocyte-macrophage colony-stimulating factor (GM-CSF) thus far. We show that the supernatant of two human colorectal carcinoma cell lines, LS1034 and SW480, stimulates proliferation of GM-CSF-dependent M07e cells. The activity was constitutively secreted by LS1034 cells and could be induced by serum-free culture conditions in SW480 cells. Addition of a neutralizing anti-GM-CSF antibody completely inhibited this activity. Preabsorption with anti-GM-CSF antibody removed all M07e growth-stimulating activity from LS1034 and SW480 supernatant. Western blot analysis revealed the presence of GM-CSF in LS1034 supernatant. Our results indicate that human colorectal carcinoma cells secrete indeed biologically active GM-CSF.
结肠癌细胞分泌多种细胞因子已得到证实。迄今为止，这些细胞因子中不包括粒细胞-巨噬细胞集落刺激因子（GM-CSF）。我们发现，两种人结肠癌细胞系LS1034和SW480的上清液可刺激依赖GM-CSF的M07e细胞增殖。该活性由LS1034细胞组成性分泌，在SW480细胞中可由无血清培养条件诱导产生。加入中和性抗GM-CSF抗体可完全抑制该活性。用抗GM-CSF抗体预吸附可去除LS1034和SW480上清液中所有的M07e生长刺激活性。蛋白质印迹分析显示LS1034上清液中存在GM-CSF。我们的结果表明，人结肠癌细胞确实分泌具有生物活性的GM-CSF。
