Hoelscher S R, Ascoli M
Department of Pharmacology, University of Iowa College of Medicine, Iowa City 52242.
Endocrinology. 1994 Aug;135(2):662-6. doi: 10.1210/endo.135.2.8033814.
Although hCG and mouse epidermal growth factor activate different signaling systems in a clonal strain of Leydig tumor cells (designated MA-10), both compounds are capable of increasing steroidogenesis, decreasing transcription of the LH/CG receptor gene, and attenuating adenylyl cyclase. We have recently identified a 21-kilodalton protein (p21) that is a potential intermediary in the actions of mouse epidermal growth factor and hCG. Characterization of p21 revealed similarities to stathmin, a ubiquitous phosphoprotein whose phosphorylation is associated with diverse second messenger pathways. Using two different experimental approaches, Western blotting and immunoprecipitation, we present data that identify p21 as stathmin.
虽然人绒毛膜促性腺激素(hCG)和小鼠表皮生长因子在一种克隆的睾丸间质细胞瘤细胞系(命名为MA - 10)中激活不同的信号系统,但这两种化合物都能够增加类固醇生成、降低促黄体生成素/绒毛膜促性腺激素(LH/CG)受体基因的转录,并减弱腺苷酸环化酶的活性。我们最近鉴定出一种21千道尔顿的蛋白质(p21),它可能是小鼠表皮生长因子和hCG作用的中介物。对p21的特性分析显示,它与一种普遍存在的磷蛋白——微管相关蛋白(stathmin)相似,其磷酸化与多种第二信使途径有关。我们使用两种不同的实验方法,即蛋白质印迹法和免疫沉淀法,提供的数据将p21鉴定为微管相关蛋白。