通过酶分析手动测定全血中红细胞内的2,3-二磷酸甘油酸，并与离子交换色谱法进行比较。

Manual determination of intra-erythrocytic 2,3-diphosphoglycerate in whole blood by enzymatic analysis, and comparison with ion-exchange chromatography.

作者信息

Detter J C, Gibson D F, MacMillan S F, Oas T H

出版信息

Clin Chem. 1975 Mar;21(3):376-80.

Abstract

A manual modification of an automated method [Atkinson, K. F., Clin. Chem. 18, 1001 (1972)] for enzymatic assay of 2,3-diphosphoglycerate is described, which is suitable for small laboratories. Samples are easily prepared for analysis, and preparations are stable for several months. Virtually all of the color generated in the colorimetric assay is produced by the diphosphoglycerate-catalyzed reaction. The coefficient of variation for multiple samples from a single subject was 2.4%. Delayed preparation of samples, particularly samples from some acidotic subjects, is shown to result in altered results. The enzyme method is compared with analysis by ion-exchange chromatography.

摘要

本文描述了一种对用于2,3-二磷酸甘油酸酶促测定的自动化方法[阿特金森，K.F.，《临床化学》18，1001（1972）]的手动改进方法，该方法适用于小型实验室。样品易于制备用于分析，且制剂可稳定保存数月。比色测定中产生的几乎所有颜色均由二磷酸甘油酸催化的反应产生。来自单个受试者的多个样品的变异系数为2.4%。结果表明，样品制备延迟，特别是来自一些酸中毒受试者的样品，会导致结果改变。将酶法与离子交换色谱分析进行了比较。