一种改进的红细胞2,3-二磷酸甘油酸测量自动化方法。
An improved automated method for the measurement of red cell 2,3-diphosphoglycerate.
作者信息
Purcell Y, Brozović B
出版信息
J Clin Pathol. 1976 Dec;29(12):1064-7. doi: 10.1136/jcp.29.12.1064.
A modified automated colorimetric micromethod for the determination of red cell 2,3-diphosphoglycerate (2,3-DPG) adapted from that of Grisolia et al (1969) is described. In the modified method, ethylenediaminetetra-acetic acid (EDTA) is not used and consequently concentrations of several reagents are changed. During the development of the method it was found that the presence of EDTA, either in the blood or in reagents, consistently reduced the measured value of 2,3-DPG by 15%. This effect of EDTA, not previously recognized, is independent of the EDTA concentration within the range of 5 to 50 mmol/1 and is at present unexplianed. In normal subjects (41 men and 30 women) the mean red cell 2,3-DPG was 14-5 mol/g haemoglobin (range 12-1-18-1 mol/g haemoglobin). There was no significant difference in 2,3-DPG concentrations between male and female subjects.
本文描述了一种改进的自动比色微量法，用于测定红细胞2,3 - 二磷酸甘油酸（2,3 - DPG），该方法改编自Grisolia等人（1969年）的方法。在改进后的方法中，不再使用乙二胺四乙酸（EDTA），因此几种试剂的浓度发生了变化。在该方法的开发过程中发现，血液或试剂中存在EDTA会使2,3 - DPG的测量值持续降低15%。EDTA的这种作用以前未被认识到，在5至50 mmol/1的范围内与EDTA浓度无关，目前尚无法解释。在正常受试者（41名男性和30名女性）中，红细胞2,3 - DPG的平均值为14 - 5 mol/g血红蛋白（范围为12 - 1 - 18 - 1 mol/g血红蛋白）。男性和女性受试者的2,3 - DPG浓度没有显著差异。
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