Prieels J P, Poortmans J, Dolmans M, Léonis J
Eur J Biochem. 1975 Jan 15;50(3):523-7. doi: 10.1111/j.1432-1033.1975.tb09892.x.
Four rabbit antibodies have been prepared, which are specifically directed against alpha-lactalbumins from different sources; namely human, cow, goat and sheep milk. Each of these antibodies was tested for its ability to react with, separately, each of the four proteins. The immunological reactions were assessed by means of different techniques: double immunodiffusion in agar gel as well as affinity chromatography of antibodies, using antigens covalently bound to an insoluble matrix. In each case, the strongest reaction was observed between homologous antibody and (matrix-bound) antigen; heterologous antigens were, however, also capable of cross-reaction. Whereas no cross-reaction between human alpha-lactalbumin and antibodies against the bovine protein could be evidenced by immunodiffusion, the occurrence of soluble complexes has been disclosed by means of a gel filtration technique.
已经制备了四种兔抗体，它们分别针对来自不同来源的α-乳白蛋白，即人乳、牛乳、山羊乳和绵羊乳中的α-乳白蛋白。对每种抗体分别检测了其与这四种蛋白质发生反应的能力。通过不同技术评估免疫反应：琼脂凝胶中的双向免疫扩散以及抗体的亲和层析，使用与不溶性基质共价结合的抗原。在每种情况下，同源抗体与（基质结合的）抗原之间观察到最强反应；然而，异源抗原也能够发生交叉反应。虽然通过免疫扩散未证明人α-乳白蛋白与抗牛蛋白抗体之间存在交叉反应，但通过凝胶过滤技术揭示了可溶性复合物的存在。
