Stevens U, Laurence D J, Ormerod M G
Clin Chim Acta. 1978 Jul 1;87(1):149-57. doi: 10.1016/0009-8981(78)90069-4.
Two radioimmunoassays for human lactalbumin have been established using a rabbit antiserum. One assay uses a second antibody to separate bound from free label; the other uses polyethylene glycol to precipitate gamma globulin non-specifically. We have confirmed that about half the normal human population have a substance in their blood which inhibits the binding of lactalbumin to the rabbit antibody. Comparison of the two assays has demonstrated that this material is not lactalbumin but a naturally occurring antibody. We have shown that it is in the IgG fraction of human plasma and is probably a cross-reacting antibody to bovine lactalbumin. None out of fifteen males and fourteen out of fifty eight non-pregnant, non-lactating females had low levels of lactalbumin in the their blood (0.6--2.0 ng/ml). Our assay could not detect a statistically significant difference between normal women and those with either benign breast disease or metastatic mammary carcinoma.
利用兔抗血清建立了两种检测人乳白蛋白的放射免疫分析法。一种分析法使用第二抗体来分离结合型和游离型标记物；另一种分析法使用聚乙二醇非特异性沉淀γ球蛋白。我们已经证实，约一半的正常人群血液中有一种物质会抑制乳白蛋白与兔抗体的结合。对这两种分析法的比较表明，这种物质不是乳白蛋白，而是一种天然存在的抗体。我们已经表明，它存在于人血浆的IgG组分中，可能是一种与牛乳白蛋白发生交叉反应的抗体。15名男性和58名非孕、非哺乳期女性中无一例血液中乳白蛋白水平较低（0.6 - 2.0 ng/ml）。我们的分析未能检测出正常女性与患有良性乳腺疾病或转移性乳腺癌的女性之间存在统计学上的显著差异。
