Marin J, Ursic-Vrscaj M, Erzen M
Institute of Microbiology, Medical Faculty of Ljubljana, Slovenia.
Isr J Med Sci. 1994 May-Jun;30(5-6):448-50.
The rate of human papillomaviruses (HPV) 16 and 18 infections were measured in 109 women with histologically or cytologically determined lesions of the uterine cervix and in 42 healthy women. Cervical swabs were taken as the source of the target viral DNA. In situ hybridization with biotinylated probes was used. HPV-16 was the predominant type in patients and in healthy women. The percentage of positive cases was the highest in cervical cancer patients: 43.3% in squamous cell carcinoma and 33.3% in adenocarcinoma followed by cervical intraepithelial neoplasia (CIN), III, II (21.4%), CIN I (14.3%) and low grade squamous intraepithelial lesions (13.6%). HPV-18 type was detected in a lower percentage in the three groups of patients. In healthy women HPV-16 was determined in 12% and HPV-18 in 4.8%. We believe that the described noninvasive method of obtaining clinical material should be the method of choice for estimating papillomavirus infections in patients and in the general population. Our results are in agreement with suggestions that HPV genotype could be an important prognostic indicator in cervical carcinoma.
对109名经组织学或细胞学确诊患有子宫颈病变的女性以及42名健康女性进行了人乳头瘤病毒（HPV）16型和18型感染率的检测。以宫颈拭子作为目标病毒DNA的来源。采用生物素化探针进行原位杂交。HPV-16是患者和健康女性中的主要类型。宫颈癌患者的阳性病例百分比最高：鳞状细胞癌中为43.3%，腺癌中为33.3%，其次是宫颈上皮内瘤变（CIN）Ⅲ级、Ⅱ级（21.4%）、CINⅠ级（14.3%）和低级别鳞状上皮内病变（13.6%）。三组患者中检测到HPV-18型的百分比更低。在健康女性中，HPV-16的检出率为12%，HPV-18的检出率为4.8%。我们认为，所描述的获取临床材料的非侵入性方法应成为评估患者和普通人群乳头瘤病毒感染的首选方法。我们的结果与HPV基因型可能是宫颈癌重要预后指标的观点一致。
