Perrin C, Baran R
Department of Pathology, Centre Hospitalier et Universitaire, Nice, France.
J Am Acad Dermatol. 1994 Aug;31(2 Pt 2):311-6. doi: 10.1016/s0190-9622(94)70161-x.
Two patients had black pigmentation affecting a great toenail that clinically simulated longitudinal melanonychia; Trichophyton rubrum with a diffusible black pigment was consistently isolated from the nail lesions. Light microscopic examination of nail samples stained with hematoxylin-eosin-safranine showed hyphae containing a brown cytoplasmic pigment. The positive Masson-Fontana stain suggested that the pigment was related to melanin. Both histologic and ultrastructural studies demonstrated intracytoplasmic polymerization, a method of final enzymatic polymerization that is unusual for melanin-producing fungal strains.
两名患者的大脚趾指甲出现黑色色素沉着，临床上类似纵向黑甲；从指甲病变中始终分离出带有可扩散黑色色素的红色毛癣菌。对苏木精-伊红-番红染色的指甲样本进行光学显微镜检查，显示菌丝含有棕色细胞质色素。马松-丰塔纳染色阳性表明该色素与黑色素有关。组织学和超微结构研究均显示有胞质内聚合现象，这是一种最终酶促聚合的方法，在产生黑色素的真菌菌株中并不常见。
