半抗原单克隆抗体的酶联免疫吸附测定筛选：半抗原-蛋白质缀合物化学结构的影响
ELISA screening of monoclonal antibodies to haptens: influence of the chemical structure of hapten-protein conjugates.
作者信息
Danilova N P
机构信息
Laboratory of Hybridomas, Institute of Biotechnology, Moscow, Russia.
出版信息
J Immunol Methods. 1994 Jul 12;173(1):111-7. doi: 10.1016/0022-1759(94)90288-7.
An analysis has been made of the influence of the chemical structure of hapten-protein conjugates on ELISA based screening monoclonal antibodies to haptens. Evidence has been obtained that the size of haptens should be taken into account. For haptens with a relatively large molecular weight, one can use a conjugate with a carrier protein differing from the immunogen. For small haptens, the screening should be conducted with a conjugate differing from the immunogen with respect to both the carrier protein and the chemical linkage between the hapten and the protein. In addition, the hapten-protein coupling should involves different amino acid residues.
已对半抗原 - 蛋白质缀合物的化学结构对基于酶联免疫吸附测定（ELISA）筛选针对半抗原的单克隆抗体的影响进行了分析。已获得证据表明，应考虑半抗原的大小。对于分子量相对较大的半抗原，可以使用与免疫原不同的载体蛋白缀合物。对于小分子半抗原，筛选应使用在载体蛋白以及半抗原与蛋白质之间的化学连接方面都与免疫原不同的缀合物。此外，半抗原 - 蛋白质偶联应涉及不同的氨基酸残基。
Zotero 插件