Baus E, Urbain J, Leo O, Andris F
Laboratoire de Physiologie Animale, Université Libre de Bruxelles, Rhode Saint-Genèse, Belgium.
J Immunol Methods. 1994 Jul 12;173(1):41-7. doi: 10.1016/0022-1759(94)90281-x.
A method is described to facilitate flow cytometric analysis of calcium mobilization upon stimulation of murine T cell hybrids. In these transformed cell lines, the accuracy of cytometric measurement of free cytoplasmic calcium with Fluo-3 is compromised by the rapid loss of the intracellular dye. We have found that the addition of sulfinpyrazone, a known organic-anion transporter inhibitor in epithelial cells and in macrophages, severely impairs the leakage of the Fluo-3 probe from the cytoplasmic matrix. Under appropriate conditions, sulfinpyrazone has little effect on the cell physiology and permits the detection of calcium influx in a variety of murine T cell hybrids.
本文描述了一种有助于对受刺激的小鼠T细胞杂交体进行钙动员流式细胞术分析的方法。在这些转化细胞系中，用Fluo-3对游离细胞质钙进行细胞计数测量的准确性因细胞内染料的快速流失而受到影响。我们发现，加入亚磺吡唑（一种已知的上皮细胞和巨噬细胞中的有机阴离子转运体抑制剂）会严重损害Fluo-3探针从细胞质基质中的泄漏。在适当条件下，亚磺吡唑对细胞生理学影响很小，并允许检测多种小鼠T细胞杂交体中的钙内流。
