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Quantitative analysis of calcium (Ca2+) mobilization after stimulation with mitogens or anti-CD3 antibodies. Simultaneous fluo-3 and immunofluorescence flow cytometry.

作者信息

Sei Y, Arora P K

机构信息

Laboratory of Neuroscience, NIDDK, National Institutes of Health, Bethesda, MD 20892.

出版信息

J Immunol Methods. 1991 Mar 21;137(2):237-44. doi: 10.1016/0022-1759(91)90029-f.

DOI:10.1016/0022-1759(91)90029-f
PMID:1672879
Abstract

A method is described for flow microfluorometric analysis of calcium mobilization in immune cells. This method is based on dual-color analysis of heterogeneous cell populations using fluo-3 and phycoerythrin (PE)-conjugated monoclonal antibody (mAb). This technique allows the detection of fluo-3 fluorescence as a measure of an increase in cytoplasmic free calcium ([Ca2+]i) while simultaneously discriminating PE-mAb-labeled cells without using wavelength ratio imaging. This method provides a unique way to distinguish patterns of calcium mobilization within a heterogeneous cell population.

摘要

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