Kobayashi H, O'Briain D S, Puri P
Children's Research Centre, Our Lady's Hospital for Sick Children, Dublin, Ireland.
J Pediatr Surg. 1994 May;29(5):651-4. doi: 10.1016/0022-3468(94)90733-1.
Nerve supporting cells were examined immunohistochemically for the expression of S-100 protein and D7 antigen (markers of peripheral Schwann cells) and GFAP (a marker of central-type nerve supporting cells) in pyloric tissue from 18 patients with infantile hypertrophic pyloric stenosis (IHPS) and 10 age-matched controls. In both the IHPS and control groups there was strong staining of the myenteric plexus by all three antibodies. Within the normal pyloric muscle, there was strong staining of nerve fibers by S-100 and D7, but fibers positive for GFAP were considerably less frequent. In the IHPS cases, S-100, D7, and GFAP immunoreactive fibers were either absent or markedly reduced within the hypertrophied circular and longitudinal muscles. Because supporting nerve cells are essential in the maintenance of basic physiological functions of neurons, their absence within the hypertrophied pyloric muscle may be an important factor in the pathogenesis of IHPS.
对18例婴儿肥厚性幽门狭窄（IHPS）患者和10例年龄匹配的对照者的幽门组织进行免疫组织化学检查，以检测神经支持细胞中S-100蛋白、D7抗原（外周施万细胞标志物）和GFAP（中枢型神经支持细胞标志物）的表达。在IHPS组和对照组中，所有三种抗体均对肌间神经丛有强烈染色。在正常幽门肌内，S-100和D7对神经纤维有强烈染色，但GFAP阳性纤维的频率要低得多。在IHPS病例中，肥厚的环形肌和纵形肌内S-100、D7和GFAP免疫反应性纤维要么缺失，要么明显减少。由于支持神经细胞对维持神经元的基本生理功能至关重要，因此它们在肥厚的幽门肌内缺失可能是IHPS发病机制中的一个重要因素。
