Lopez-Neblina F, Toledo-Pereyra L H, Suzuki S
Surgical Research Institute, Kalamazoo, MI.
Microsurgery. 1994;15(4):274-8. doi: 10.1002/micr.1920150411.
An effective and ultrarapid technique for kidney transplantation in the rat is introduced. In this manner, physiological and immunological parameters could be tested without difficulty in small animals such as the rat. Shorter periods of warm ischemia were achieved by rapid harvesting and anastomotic times using a combination of the sleeve technique for the artery, a cuff technique for the vein, and the over-a-stem technique for the ureter. In 20 rats, total vascular (artery and vein) anastomotic times were 5.35 +/- 0.59 min, of which the artery was 4.30 +/- 0.40 minutes and the vein was 1.05 +/- 0.20 min. The ureter anastomotic time was 1.0 +/- 0.1 min. The surgical complications were three ureteral stenoses. The 15 day survival was 100%. Plasma creatinine and histological findings at 24 and 30 days after transplantation were within normal limits with no evidence of ischemic damage. The advantage of this technique is its effectivity and simplicity that allows for reduced times of warm ischemia. This is the fastest technique published so far in the literature. We propose this technique to be employed in all studies of kidney transplantation and preservation in the rat.
介绍了一种用于大鼠肾移植的有效且超快速的技术。通过这种方式，可以在大鼠等小动物中轻松测试生理和免疫参数。采用动脉袖套技术、静脉袖带技术和输尿管套杆技术相结合的方法，通过快速摘取和吻合时间实现了较短的热缺血时间。在20只大鼠中，总的血管（动脉和静脉）吻合时间为5.35±0.59分钟，其中动脉为4.30±0.40分钟，静脉为1.05±0.20分钟。输尿管吻合时间为1.0±0.1分钟。手术并发症为3例输尿管狭窄。15天存活率为100%。移植后24天和30天的血浆肌酐和组织学检查结果均在正常范围内，无缺血损伤迹象。该技术的优点是有效性和简单性，可减少热缺血时间。这是迄今为止文献中报道的最快的技术。我们建议在所有大鼠肾移植和保存研究中采用该技术。
