Jin Z-D, Xue L-N, Peng L-S
Department of Kidney Center, Guangdong Provincial Hospital of Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong Province, People's Republic of China.
Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong Province, People's Republic of China.
Transplant Proc. 2017 Oct;49(8):1942-1946. doi: 10.1016/j.transproceed.2017.04.022.
We describe a simple and reliable orthotopic kidney transplantation method in rats with the use of sleeve arterial anastomosis and a modified stenting technique for anastomosis of the renal vein (RV).
Male Fischer and Lewis rats were used as kidney donors and recipients, respectively, and their left kidneys were harvested in situ. In the control rats (n = 30), the renal artery (RA) and RV anastomoses were performed end-to-end with interrupted sutures by means of the conventional technique. In the experimental animals (n = 30), revascularization of the RA was fashioned end-in-end with the use of a modified sleeve anastomosis, the RV was anastomosed end-to-end with the use of a modified stenting technique and interrupted sutures, and the ureter was anastomosed with the use of the end-to-end interrupted suture technique.
The arterial anastomosis time in the control group was 8.52 ± 1.1 minutes, and that in the experimental group was 4.7 ± 0.6 minutes (P < .05). The venous anastomosis time in the experimental group was 9.2 ± 1.3 minutes, which also was less than in the control group (11.19 ± 0.78 minutes; P < .05). The warm ischemia time decreased from 26.8 ± 1.3 minutes in the control group to 20.7 ± 0.5 minutes in the experimental group (P < .05). The success rate of 93% at 21 days after grafting was identical in the experimental and control groups.
We developed a modified model of orthotopic kidney transplantation that can significantly reduce the warm ischemia time.
我们描述了一种在大鼠中使用袖套动脉吻合术和改良支架技术进行肾静脉（RV）吻合的简单可靠的原位肾移植方法。
分别使用雄性Fischer大鼠和Lewis大鼠作为供体和受体，原位摘取它们的左肾。在对照组大鼠（n = 30）中，采用传统技术通过间断缝合进行肾动脉（RA）和RV端端吻合。在实验组动物（n = 30）中，采用改良袖套吻合术进行RA端对端血管重建，采用改良支架技术和间断缝合进行RV端端吻合，并采用端端间断缝合技术进行输尿管吻合。
对照组的动脉吻合时间为8.52±1.1分钟，实验组为4.7±0.6分钟（P <.05）。实验组的静脉吻合时间为9.2±1.3分钟，也短于对照组（11.19±0.78分钟；P <.05）。热缺血时间从对照组的26.8±1.3分钟降至实验组的20.7±0.5分钟（P <.05）。实验组和对照组在移植后21天的成功率均为93%。
我们开发了一种改良的原位肾移植模型，可显著减少热缺血时间。
