Fleckenstein B, Bornkamm G W, Ludwig H
J Virol. 1975 Feb;15(2):398-406. doi: 10.1128/JVI.15.2.398-406.1975.
Two types of Herpesvirus saimiri genomes can be isolated from purified virions: (i) the M genome is a double-stranded, liniear DNA molecule with a mean contour length corresponding to 89 times 10-6 daltons. The M genome contains about 70% of unique sequences (light DNA, 36% guanine plus cytosine) and 30% reiterated sequences (heavy DNA, 71% guanine plus cytosine). (ii) the H genome is composed of heavy DNA only and is more heterogeneous in size. The sequences in the H genome are up to 40-fold reiterated, indicating defectiveness of this type of genome. The repetitions in the H genome and the M genome cross-hybridize almost completely and have identical kinetic complexity (2.8 times 10-6 daltons). DNA infectivity studies by using the calcium phosphate and the DEAE-dextran method gave further evidence that H genomes are defective: no infectious virus was recovered from permissive cells treated with heavy DNA, whereas M genome-infected cells developed cytopathic changes after 11 to 56 days. Defective H genomes were present in the progeny virus two passages after transfection.
（i）M基因组是一种双链线性DNA分子，其平均轮廓长度相当于89×10⁻⁶道尔顿。M基因组包含约70%的独特序列（轻DNA，鸟嘌呤加胞嘧啶占36%）和30%的重复序列（重DNA，鸟嘌呤加胞嘧啶占71%）。（ii）H基因组仅由重DNA组成，且大小更具异质性。H基因组中的序列重复达40倍，表明这种类型的基因组存在缺陷。H基因组和M基因组中的重复序列几乎完全交叉杂交，且具有相同的动力学复杂度（2.8×10⁻⁶道尔顿）。使用磷酸钙和DEAE - 葡聚糖方法进行的DNA感染性研究进一步证明H基因组存在缺陷：用重DNA处理的允许细胞未回收感染性病毒，而感染M基因组的细胞在11至56天后出现细胞病变变化。转染后两代子代病毒中存在缺陷的H基因组。
