Goltz M, Broll H, Mankertz A, Weigelt W, Ludwig H, Buhk H J, Borchers K
Robert Koch-Institut, Freien Universität Berlin, Germany.
Virus Genes. 1994 Sep;9(1):53-9. doi: 10.1007/BF01703435.
In order to estimate the phylogenetic relationship of BHV-4 among the herpesviruses, we have cloned and sequenced its glycoprotein B (gB). The 2.6 kb open reading frame codes for a 874 amino acid long protein. The comparison of its deduced amino acid sequence with those of its counterparts in 19 distinct herpesviruses groups BHV-4 into the gamma-herpesvirinae. The calculation of an evolutionary tree emphasized that BHV-4 is more closely related to herpesvirus saimiri (HVS) than to Epstein-Barr virus (EBV). However, in contrast to EBV and HVS, the gB of BHV-4 contains a putative protease cleavage site and 20 potential N-glycosylation sites. The alignment of the amino acid sequences revealed that 10 cysteine and 7 proline residues, as well as the motifs SPF and GQLG, were completely conserved among the 20 investigated gBs.
为了评估牛疱疹病毒4型（BHV-4）在疱疹病毒中的系统发育关系，我们克隆并测序了其糖蛋白B（gB）。这个2.6 kb的开放阅读框编码一个由874个氨基酸组成的蛋白质。将其推导的氨基酸序列与19种不同疱疹病毒组中的对应序列进行比较后，BHV-4被归入γ-疱疹病毒亚科。进化树的计算结果表明，BHV-4与猴疱疹病毒（HVS）的关系比与爱泼斯坦-巴尔病毒（EBV）更为密切。然而，与EBV和HVS不同的是，BHV-4的gB含有一个假定的蛋白酶切割位点和20个潜在的N-糖基化位点。氨基酸序列比对显示，在20种被研究的gB中，10个半胱氨酸和7个脯氨酸残基以及基序SPF和GQLG是完全保守的。
