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Properties of initiator-associated transcription mediated by GAL4-VP16.

作者信息

Chang C, Gralla J D

机构信息

Department of Chemistry and Biochemistry, University of California, Los Angeles 90024-1569.

出版信息

Mol Cell Biol. 1993 Dec;13(12):7469-75. doi: 10.1128/mcb.13.12.7469-7475.1993.

DOI:10.1128/mcb.13.12.7469-7475.1993
PMID:8246964
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC364818/
Abstract

Transcription associated with a terminal deoxynucleotide transferase gene initiator element is shown to respond to the transcription factor GAL4-VP16 both in vivo and in vitro. High-level transcription requires both an intact initiator element and bound activator. Transcription from this initiator-directed promoter is synergistic in vivo in that five GAL4 DNA binding sites yield 36 times the expression of a single site. Promoters dominated by initiator and TATA elements respond similarly to several GAL4-based activators, including GAL4-Sp1, GAL4-CTF, GAL4(1-147), GAL4-p53, GAL4-C/EBP, and GAL4-ER(EF), as well as GAL4-VP16 and Sp1. These and other similarities suggest that primary activation of TATA- and initiator-dominated promoters occurs at common steps. Since the initial assembly steps do not appear to be common for the two promoter types, the results place interesting constraints on models for how activation occurs.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1452/364818/f240b54dfff0/molcellb00024-0284-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1452/364818/96bdd5b5b7de/molcellb00024-0283-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1452/364818/f240b54dfff0/molcellb00024-0284-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1452/364818/96bdd5b5b7de/molcellb00024-0283-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1452/364818/f240b54dfff0/molcellb00024-0284-a.jpg

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Hepatocyte nuclear factor 4alpha enhances the hepatocyte nuclear factor 1alpha-mediated activation of transcription.肝细胞核因子4α增强肝细胞核因子1α介导的转录激活。
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The activity of the activation function 2 of the human hepatocyte nuclear factor 4 (HNF-4alpha) is differently modulated by F domains from various origins.

本文引用的文献

1
A common ATP requirement for open complex formation and transcription at promoters containing initiator or TATA elements.对于含有起始子或TATA元件的启动子，开放复合物形成和转录存在共同的ATP需求。
J Biol Chem. 1993 Mar 25;268(9):6535-40.
2
Synergism in transcriptional activation: a kinetic view.转录激活中的协同作用：动力学视角
Genes Dev. 1993 Feb;7(2):173-9. doi: 10.1101/gad.7.2.173.
3
Three in one and one in three: it all depends on TBP.三位一体与一分为三：一切都取决于TBP。
人肝细胞核因子4（HNF - 4α）的激活功能2的活性受到来自不同来源的F结构域的不同调节。
Biochem J. 1999 May 15;340 ( Pt 1)(Pt 1):161-9.
4
Conservation and divergence of NF-Y transcriptional activation function.NF-Y转录激活功能的保守性与差异性
Nucleic Acids Res. 1998 Aug 15;26(16):3800-5. doi: 10.1093/nar/26.16.3800.
5
Corepressor SMRT binds the BTB/POZ repressing domain of the LAZ3/BCL6 oncoprotein.共抑制因子SMRT与LAZ3/BCL6癌蛋白的BTB/POZ抑制结构域结合。
Proc Natl Acad Sci U S A. 1997 Sep 30;94(20):10762-7. doi: 10.1073/pnas.94.20.10762.
6
The ETS family member ERM contains an alpha-helical acidic activation domain that contacts TAFII60.ETS家族成员ERM包含一个与TAFII60接触的α螺旋酸性激活结构域。
Nucleic Acids Res. 1997 Nov 15;25(22):4455-63. doi: 10.1093/nar/25.22.4455.
7
Transcription reinitiation rate: a special role for the TATA box.转录重新起始率：TATA 框的特殊作用。
Mol Cell Biol. 1997 Jul;17(7):3809-16. doi: 10.1128/MCB.17.7.3809.
8
Position-dependent transcriptional regulation of the murine dihydrofolate reductase promoter by the E2F transactivation domain.E2F反式激活结构域对小鼠二氢叶酸还原酶启动子的位置依赖性转录调控
Mol Cell Biol. 1997 Apr;17(4):1966-76. doi: 10.1128/MCB.17.4.1966.
9
The initiator element of the adenovirus major late promoter has an important role in transcription initiation in vivo.腺病毒主要晚期启动子的起始元件在体内转录起始过程中起重要作用。
J Virol. 1997 Jan;71(1):102-9. doi: 10.1128/JVI.71.1.102-109.1997.
10
Transcription activation by GC-boxes: evaluation of kinetic and equilibrium contributions.GC盒介导的转录激活：动力学和平衡贡献的评估
Nucleic Acids Res. 1996 Jul 15;24(14):2723-9. doi: 10.1093/nar/24.14.2723.
Cell. 1993 Jan 15;72(1):7-10. doi: 10.1016/0092-8674(93)90042-o.
4
Identification of a transcriptional initiator element in the cytochrome c oxidase subunit Vb promoter which binds to transcription factors NF-E1 (YY-1, delta) and Sp1.细胞色素c氧化酶亚基Vb启动子中转录起始元件的鉴定，该元件可与转录因子NF-E1（YY-1，δ）和Sp1结合。
J Biol Chem. 1993 Feb 25;268(6):4188-96.
5
Mechanism of initiator-mediated transcription: evidence for a functional interaction between the TATA-binding protein and DNA in the absence of a specific recognition sequence.引发剂介导的转录机制：在缺乏特定识别序列的情况下，TATA 结合蛋白与 DNA 之间功能相互作用的证据。
Mol Cell Biol. 1993 Jul;13(7):3841-9. doi: 10.1128/mcb.13.7.3841-3849.1993.
6
Eukaryotic gene transcription with purified components.真核基因转录与纯化的组分。
Methods Enzymol. 1983;101:582-98. doi: 10.1016/0076-6879(83)01039-3.
7
Yeast mRNA initiation sites are determined primarily by specific sequences, not by the distance from the TATA element.
EMBO J. 1985 Dec 1;4(12):3273-80. doi: 10.1002/j.1460-2075.1985.tb04077.x.
8
The relationship between the "TATA" sequence and transcription initiation sites at the HIS4 gene of Saccharomyces cerevisiae.酿酒酵母HIS4基因处的“TATA”序列与转录起始位点之间的关系。
Proc Natl Acad Sci U S A. 1985 Dec;82(24):8557-61. doi: 10.1073/pnas.82.24.8557.
9
Transcription initiation of the Saccharomyces cerevisiae iso-1-cytochrome c gene. Multiple, independent T-A-T-A sequences.酿酒酵母同工-1-细胞色素c基因的转录起始。多个独立的T-A-T-A序列。
J Mol Biol. 1986 Feb 5;187(3):363-78. doi: 10.1016/0022-2836(86)90439-0.
10
Each of three "TATA elements" specifies a subset of the transcription initiation sites at the CYC-1 promoter of Saccharomyces cerevisiae.酿酒酵母CYC-1启动子处的三个“TATA元件”中的每一个都指定了转录起始位点的一个子集。
Proc Natl Acad Sci U S A. 1985 Dec;82(24):8562-6. doi: 10.1073/pnas.82.24.8562.