小鼠中与带状环蛇小卫星（Bkm）相关的Y染色体特异性重复DNA。

Banded krait minor-satellite (Bkm)-associated Y chromosome-specific repetitive DNA in mouse.

作者信息

Singh L, Panicker S G, Nagaraj R, Majumdar K C

机构信息

Centre for Cellular and Molecular Biology, Hyderabad, India.

出版信息

Nucleic Acids Res. 1994 Jun 25;22(12):2289-95. doi: 10.1093/nar/22.12.2289.

The mouse Y chromosome remains highly condensed in all somatic tissues but decondenses extensively in testis. We have isolated a mouse Y chromosome-specific repeat M34 (11.5 kb) and shown that this is distributed along the Y chromosome except the sex-determining region (the Y short arm) in which GATA repeats are predominantly concentrated. It has 32 copies of GATA repeats in a 2.7 kb fragment. About 200-300 copies of M34 on the Y chromosome are interspersed among other sequences. A 1.2 kb fragment (p3) of M34, containing GATA repeats, also has scaffold attachment region (SAR) motifs which bind to nuclear matrices. A strong affinity of histone H1 to SAR motifs is implicated in maintaining the condensed state of the Y chromosome in somatic tissues. The probable significance of molecular organization of the Y chromosome is discussed.

小鼠Y染色体在所有体细胞组织中都保持高度浓缩状态，但在睾丸中会大量解聚。我们分离出了一种小鼠Y染色体特异性重复序列M34（11.5 kb），并表明它分布在整个Y染色体上，除了性别决定区域（Y染色体短臂），该区域主要集中了GATA重复序列。它在一个2.7 kb的片段中有32个GATA重复序列拷贝。Y染色体上约200 - 300个M34拷贝散布在其他序列之间。M34的一个1.2 kb片段（p3），包含GATA重复序列，也具有与核基质结合的支架附着区域（SAR）基序。组蛋白H1与SAR基序的强亲和力与维持体细胞组织中Y染色体的浓缩状态有关。文中讨论了Y染色体分子组织的可能意义。