Erickson R P, Ross C E, Gorski J L, Stalvey J R, Drumm M M
Department of Human Genetics, University of Michigan School of Medicine, Ann Arbor 48109-0618.
Ann Hum Genet. 1988 Jul;52(3):167-76. doi: 10.1111/j.1469-1809.1988.tb01094.x.
In order to determine whether the regional localizations of Bkm repeats detected on the human X chromosome consisted of typical GATA/GACA repeats, clones were isolated, mapped, and sequenced. Nine Bkm-hybridizing clones from Kunkel's fluorescent-activated, cell-sorted X-chromosome library were all unique. Five were mapped in detail with restriction enzymes and the Bkm-hybridizing segments were localized. Confirmation of X chromosomal homology was obtained for 2 of the clones and Bkm segments from these 2 clones were sequenced. Seventeen contiguous GATA repeats were found in each clone and the overall repeat arrangement showed relatively few differences from previously sequenced Bkm sequences. These are the first sequences of human Bkm repeats. The results, when compared with previously published results, suggest that there may be significant differences between the organization of Bkm repeats on the human X and on the human Y chromosome.
为了确定在人类X染色体上检测到的Bkm重复序列的区域定位是否由典型的GATA/GACA重复序列组成，对克隆进行了分离、定位和测序。从昆克尔的荧光激活细胞分选X染色体文库中获得的9个与Bkm杂交的克隆都是独一无二的。其中5个克隆用限制性酶进行了详细定位，并确定了与Bkm杂交的片段的位置。对其中2个克隆进行了X染色体同源性确认，并对这2个克隆的Bkm片段进行了测序。在每个克隆中发现了17个连续的GATA重复序列，总体重复序列排列与先前测序的Bkm序列相比差异相对较小。这些是人类Bkm重复序列的首批序列。与先前发表的结果相比，这些结果表明人类X染色体和Y染色体上Bkm重复序列的组织可能存在显著差异。
