Lillevang S T, Georgsen J, Kristensen T
Department of Clinical Immunology, Odense University Hospital, Denmark.
Vox Sang. 1994;66(3):210-5. doi: 10.1111/j.1423-0410.1994.tb00312.x.
The recently introduced gel technique offers significantly advances compared to traditional tube tests. The purpose of the present study was to design an antibody screening test based on the gel technique, pooled cells, and plasma and to evaluate this test by comparison with a conventional spin-tube indirect antiglobulin test (IAT) combined with a two-stage papain technique. Pilot studies were performed to establish optimal parameters during the design phase, and finally 5,446 consecutive samples were screened for irregular antibodies by the gel technique in parallel with routine techniques. Irregular erythrocyte antibodies were detected in 151 samples, and the gel technique proved equal or superior to the tube test in the detection of all antibodies except 'enzyme-only' anti-E and anti-Lea. We conclude from this study that screening for unexpected antibodies using the gel IAT in our set-up, which includes: (1) omission of enzyme technique; (2) the use of stabilized (EDTA) plasma instead of serum as test material in order to facilitate automation; and (3) pooling 2 by 2 of 4 test cells (to make the gel technique price competitive), is a fast, reliable and sensitive procedure that maintains transfusion safety and compares favourably with our previous routine of saline IAT combined with a two-stage papain technique.
与传统试管检测相比，最近推出的凝胶技术有了显著进步。本研究的目的是设计一种基于凝胶技术、混合细胞和血浆的抗体筛查试验，并通过与传统的试管间接抗球蛋白试验（IAT）结合两阶段木瓜蛋白酶技术进行比较来评估该试验。在设计阶段进行了初步研究以确定最佳参数，最后对5446份连续样本同时采用凝胶技术和常规技术进行不规则抗体筛查。在151份样本中检测到不规则红细胞抗体，结果表明，除了“仅酶法”检测的抗 - E和抗 - Lea外，凝胶技术在检测所有抗体方面与试管试验相当或更优。我们从这项研究得出结论，在我们的检测方法中使用凝胶IAT筛查意外抗体，该方法包括：（1）省略酶技术；（2）使用稳定化（乙二胺四乙酸）血浆而非血清作为检测材料以利于自动化；（3）将4个检测细胞两两混合（以使凝胶技术具有价格竞争力），是一种快速、可靠且灵敏的方法，可维持输血安全，与我们之前采用的盐水IAT结合两阶段木瓜蛋白酶技术的常规方法相比具有优势。
