Yamamoto Y, Kitamura N, Yamada J, Yamashita T
Department of Veterinary Anatomy, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
Acta Anat (Basel). 1994;149(2):104-10. doi: 10.1159/000147564.
The distribution in the sheep omasum of peptide- and catecholamine-containing neurons was investigated by an immunohistochemical technique using antibodies against six neuropeptides and two catecholamine-synthesizing enzymes. In the myenteric plexus, both nerve cell bodies and nerve fibers were positively immunostained for substance P (SP), methionine enkephalin (ENK), vasoactive intestinal polypeptide (VIP), neuropeptide Y (NPY) and galanin (GAL). In contrast, immunoreactivity specific for calcitonin gene-related peptide (CGRP), tyrosine hydroxylase (TH) and dopamine-beta-hydroxylase (DBH) was detected only in nerve fibers. In the omasal wall, nerve fibers with SP- and ENK-like immunoreactivity were prominent in the outer muscle layer, while numerous nerve fibers with VIP-, NPY- and GAL-like immunoreactivity were observed in the inner muscle layer. In the lamina propria mucosae, and in particular in the omasal papillae, a dense network of peptide-containing nerves was found. The distribution of nerves with CGRP-like immunoreactivity was different from that of other immunoreactive nerves. The networks of nerves with TH-, DBH- and NPY-like immunoreactivity were concentrated around the vascular components. The results of this study indicate the complexity of the enteric nervous system in the sheep omasum, reflecting the multiple functions of the nerves in the regulation of motility and/or absorption and secretion.
采用免疫组织化学技术，使用针对六种神经肽和两种儿茶酚胺合成酶的抗体，研究了绵羊皱胃中含肽和儿茶酚胺神经元的分布。在肌间神经丛中，神经细胞体和神经纤维对P物质（SP）、甲硫氨酸脑啡肽（ENK）、血管活性肠多肽（VIP）、神经肽Y（NPY）和甘丙肽（GAL）均呈阳性免疫染色。相比之下，降钙素基因相关肽（CGRP）、酪氨酸羟化酶（TH）和多巴胺-β-羟化酶（DBH）的免疫反应性仅在神经纤维中检测到。在皱胃壁中，具有SP样和ENK样免疫反应性的神经纤维在外肌层中突出，而在内肌层中观察到许多具有VIP样、NPY样和GAL样免疫反应性的神经纤维。在固有层黏膜中，特别是在皱胃乳头中，发现了一个密集的含肽神经网络。具有CGRP样免疫反应性的神经分布与其他免疫反应性神经不同。具有TH样、DBH样和NPY样免疫反应性的神经网络集中在血管成分周围。本研究结果表明绵羊皱胃中肠神经系统的复杂性，反映了神经在调节运动和/或吸收与分泌中的多种功能。
