来自克氏锥虫糖蛋白的O-糖苷键连接的N-乙酰葡糖胺结合寡糖。
O-glycosidically linked N-acetylglucosamine-bound oligosaccharides from glycoproteins of Trypanosoma cruzi.
作者信息
Previato J O, Jones C, Gonçalves L P, Wait R, Travassos L R, Mendonça-Previato L
机构信息
Instituto de Microbiolgia CCS-bloco I, Universidade Federal do Rio de Janeiro, Brasil.
出版信息
Biochem J. 1994 Jul 1;301 ( Pt 1)(Pt 1):151-9. doi: 10.1042/bj3010151.
In this report we describe studies on the structures of the O-linked carbohydrate units in cell-surface glycoproteins of epimastigote forms of the G-strain of Trypanosoma cruzi. Mild alkaline reductive degradation of the 38/43 kDa glycoproteins resulted in beta-elimination of glycosylated threonine and/or serine residues, and the liberation of N-acetylglucosaminitol, galactobiosyl-, galactotriosyl-, galactotetraosyl- and galactopentaosyl-N-acetylglucosaminitol. The structures of these oligosaccharide alditols were established by n.m.r. spectroscopy and methylation analysis as: Galf beta 1-4(Galp beta 1-6)GlcNAc-ol; Galp beta 1-3Galp beta 1-6(Galf beta 1-4)GlcNAc-ol; [(Galp beta 1-3)(Galp beta 1-2)Galp beta 1-6](Galf beta 1-4)GlcNAc-ol; [(Galp beta 1-3)(Galp beta 1-2)Galp beta 1-6](Galp beta 1-2Galf beta 1-4)GlcNAc-ol.
在本报告中，我们描述了对克氏锥虫G株型无鞭毛体形式的细胞表面糖蛋白中O-连接碳水化合物单元结构的研究。对38/43 kDa糖蛋白进行温和碱性还原降解，导致糖基化苏氨酸和/或丝氨酸残基发生β-消除，并释放出N-乙酰葡糖胺醇、半乳糖二糖基-、半乳糖三糖基-、半乳糖四糖基-和半乳糖五糖基-N-乙酰葡糖胺醇。通过核磁共振光谱和甲基化分析确定了这些低聚糖糖醇的结构如下：Galfβ1-4（Galpβ1-6）GlcNAc-ol；Galpβ1-3Galpβ1-6（Galfβ1-4）GlcNAc-ol；[（Galpβ1-3）（Galpβ1-2）Galpβ1-6]（Galfβ1-4）GlcNAc-ol；[（Galpβ1-3）（Galpβ1-2）Galpβ1-6]（Galpβ1-2Galfβ1-4）GlcNAc-ol。
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