Hattori T, Ohsawa K, Mizuno Y, Kato K, Kohsaka S
Department of Neurochemistry, National Institute of Neuroscience, Tokyo, Japan.
Biochem Biophys Res Commun. 1994 Jul 15;202(1):25-30. doi: 10.1006/bbrc.1994.1888.
Neuron-specific enolase (NSE), one of the glycolytic enzymes, is a gamma gamma-isozyme of enolase that is specifically expressed in neurons. Our previous studies demonstrated that NSE promotes survival of rat embryonic neocortical neurons in culture but that the alpha alpha-isozyme (non-neuronal enolase; NNE) has no effect. In this study, we found that a synthetic peptide corresponding to the C-terminal portion of NSE (404-433) also promotes the survival of neocortical neurons. By contrast, a synthetic peptide of the C-terminal portion of NNE (404-433) has no effect on neuronal survival. These findings would be important for further analysis of the neurotrophic mechanism of NSE.
神经元特异性烯醇化酶（NSE）是一种糖酵解酶，是烯醇化酶的γγ同工酶，在神经元中特异性表达。我们之前的研究表明，NSE可促进培养的大鼠胚胎新皮质神经元的存活，但αα同工酶（非神经元烯醇化酶；NNE）则无此作用。在本研究中，我们发现与NSE C端部分（404 - 433）对应的合成肽也能促进新皮质神经元的存活。相比之下，NNE C端部分（404 - 433）的合成肽对神经元存活没有影响。这些发现对于进一步分析NSE的神经营养机制具有重要意义。
