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Evidence for a two proton dependent redox equilibrium in an archaeal Rieske iron-sulfur cluster.

作者信息

Anemüller S, Schmidt C L, Schäfer G, Bill E, Trautwein A X, Teixeira M

机构信息

Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Oeiras, Portugal.

出版信息

Biochem Biophys Res Commun. 1994 Jul 15;202(1):252-7. doi: 10.1006/bbrc.1994.1920.

DOI:10.1006/bbrc.1994.1920
PMID:8037720
Abstract

The recently detected Rieske iron-sulfur center in the membrane of the thermoacidophilic archaeon Sulfolobus acidocaldarius (Anemüller et al., 1993, FEBS Lett. 318, 61-64) was further characterized by EPR spectroscopy, coupled to redox-potentiometry and functional studies. The reduction potential is pH-dependent above pH 6, revealing the influence of two ionization equilibria in the oxidized form, with pKaox-values of 6.2 and 8.5. Above pH 9, the slope of the curve is--120 mV/pH-unit. A partially purified fraction exerted a ubiquinol-cytochrome c oxidoreductase activity. To our knowledge, for the first time, in a membrane bound Rieske iron-sulfur protein, unequivocal evidence for a two proton dependent redox equilibrium is presented.

摘要

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