Mikami H, Watanabe S, Hirose M, Sato N
Department of Gastroenterology, Juntendo University School of Medicine, Tokyo, Japan.
Biochem Biophys Res Commun. 1994 Jul 15;202(1):285-92. doi: 10.1006/bbrc.1994.1925.
Effects of extracellular matrix on wound repair of cultured gastric epithelial cells were assessed. Artificial wounds were made by mechanical cell denudation in confluent rabbit gastric epithelial cell sheets which were formed on different types of extracellular matrix (e.g., collagen type I and type IV, laminin, fibronectin and Matrigel). Changes in wound size were analyzed quantitatively. Cell migration and proliferation were observed in stages of the wound repair process. The speed of wound repair was different with each extracellular matrix studied and was fastest on Matrigel. The type of extracellular matrix used in this study modulated both cell migration and proliferation. Therefore, it is concluded that extracellular matrix plays an important role in rates of gastric mucosal wound healing.
评估了细胞外基质对培养的胃上皮细胞伤口修复的影响。通过机械性细胞剥脱在不同类型细胞外基质（如I型和IV型胶原、层粘连蛋白、纤连蛋白和基质胶）上形成的汇合兔胃上皮细胞片上制造人工伤口。定量分析伤口大小的变化。在伤口修复过程的各个阶段观察细胞迁移和增殖。所研究的每种细胞外基质的伤口修复速度不同，在基质胶上最快。本研究中使用的细胞外基质类型调节细胞迁移和增殖。因此，得出结论，细胞外基质在胃黏膜伤口愈合速率中起重要作用。
