Virtanen I, Tani T, Bäck N, Häppölä O, Laitinen L, Kiviluoto T, Salo J, Burgeson R E, Lehto V P, Kivilaakso E
Department of Anatomy, University of Helsinki, Finland.
Am J Pathol. 1995 Oct;147(4):1123-32.
The proliferating cells of the gastric mucosa are found among the pit and mucous neck cells. These cells migrate upward to renew the surface epithelium and downward to restitute the glandular cells. As the epithelial basement membranes (BMs) function as substrate for cell adhesion and migration as well as signals for their differentiation, we studied, by indirect immunofluorescence microscopy, the distribution of different laminin chains and their integrin receptors in adult human stomach. The immunoreactivity for laminin alpha 2 chain localized to the BMs of glands and the lower parts of the gastric pits whereas the laminin alpha 3 chain (laminin-5/kalinin) immunoreactivity was strictly confined to BMs underneath the surface epithelium and the upper parts of the pits. Proliferating mucosal epithelial cells, identified by Ki-67 antibodies, were confined to the areas containing both alpha 2 and alpha 3 laminin chains. The alpha 1, beta 1, and gamma 1 laminin chains were found in all BMs of the mucosa whereas the beta 2 chain was prominent in mucosal blood vessels and also detectable in some glands. Among the laminin integrin receptors, the alpha 3 and beta 4 subunits were seen to be expressed in cells along the BMs with the alpha 3 laminin chain. The alpha 6 integrin, on the other hand, was seen in all gastric epithelia. The present results demonstrate that in the adult human stomach laminin alpha 2 and alpha 3 chains show zonal distribution in BM underlying gastric mucosal epithelium whereas other laminin chains show a more general distribution.
胃黏膜的增殖细胞存在于胃小凹和黏液颈细胞之间。这些细胞向上迁移以更新表面上皮，向下迁移以补充腺细胞。由于上皮基底膜（BMs）作为细胞黏附和迁移的底物以及细胞分化的信号，我们通过间接免疫荧光显微镜研究了不同层粘连蛋白链及其整合素受体在成人胃中的分布。层粘连蛋白α2链的免疫反应定位于腺体和胃小凹下部的基底膜，而层粘连蛋白α3链（层粘连蛋白-5/卡利宁）的免疫反应严格局限于表面上皮下方和小凹上部的基底膜。通过Ki-67抗体鉴定的增殖性黏膜上皮细胞局限于同时含有α2和α3层粘连蛋白链的区域。α1、β1和γ1层粘连蛋白链存在于黏膜的所有基底膜中，而β2链在黏膜血管中突出，在一些腺体中也可检测到。在层粘连蛋白整合素受体中，α3和β4亚基在沿着含有α3层粘连蛋白链的基底膜的细胞中表达。另一方面，α6整合素在所有胃上皮中均可见。目前的结果表明，在成人胃中，层粘连蛋白α2和α3链在胃黏膜上皮下方的基底膜中呈区域分布，而其他层粘连蛋白链则呈更普遍的分布。
