Walter R, Schaffner A, Schoedon G
Department of Medicine, University Hospital of Zürich, Switzerland.
Biochem Biophys Res Commun. 1994 Jul 15;202(1):450-5. doi: 10.1006/bbrc.1994.1949.
The murine vascular endothelial cell line send1 expresses both constitutive and inducible nitric oxide (NO) synthases. Interferon-gamma (IFN gamma) or endotoxin (LPS) alone inhibited constitutive NO production in a dose and time dependent manner. Addition of L-arginine had no influence on the decrease of NO production caused by IFN gamma or LPS. On the other hand, IFN gamma and LPS synergized in the induction of high output NO production. Successive incubations with IFN gamma and LPS in different sequences revealed IFN gamma as the time setting signal for the induction of NO synthesis. These results demonstrate that LPS and IFN gamma have a direct suppressive effect on constitutive NO synthase while at the same time they strongly activate inducible NO production. Thus inflammatory stimuli trigger murine vascular endothelial cells to switch from constitutive to inducible NO synthase activity.
小鼠血管内皮细胞系send1表达组成型和诱导型一氧化氮（NO）合酶。单独使用干扰素-γ（IFNγ）或内毒素（LPS）以剂量和时间依赖性方式抑制组成型NO的产生。添加L-精氨酸对IFNγ或LPS引起的NO产生减少没有影响。另一方面，IFNγ和LPS在诱导高产量NO产生方面具有协同作用。以不同顺序先后用IFNγ和LPS孵育表明，IFNγ是诱导NO合成的时间设定信号。这些结果表明，LPS和IFNγ对组成型NO合酶具有直接抑制作用，同时它们强烈激活诱导型NO的产生。因此，炎症刺激触发小鼠血管内皮细胞从组成型NO合酶活性转变为诱导型NO合酶活性。
