Ding M, Beck R J, Keller C J, Fenton R G
Clinical Research Branch, NCI-FCRDC, MD 21702.
Biochem Biophys Res Commun. 1994 Jul 15;202(1):549-55. doi: 10.1006/bbrc.1994.1963.
The spectrum of MAGE gene expression in the human melanoma cell line DM150 was examined using reverse transcription polymerase chain reaction and cDNA cloning. We have isolated five full-length cDNAs from DM150 which were identified as MAGE-1, MAGE-3, MAGE-12 and two previously undescribed MAGE genes, MAGE-3b and MAGE-X2. DNA sequence analysis of the coding regions of the MAGE-3b and MAGE-X2 genes revealed 83% and 88% identity with MAGE-1, while MAGE-3b was 98% homologous with the full length MAGE-3 clone. The predicted amino acid sequences of MAGE-X2 and MAGE-3b contain consensus HLA-A1 peptide binding motifs, suggesting that, like MAGE-1, they may code for tumor-associated antigens. In addition, a nonamer peptide encoded by both the MAGE-3 and MAGE-12 genes was shown by direct binding studies to contain an aggretope for HLA-A2.
利用逆转录聚合酶链反应和cDNA克隆技术，对人黑色素瘤细胞系DM150中MAGE基因的表达谱进行了检测。我们从DM150中分离出了五个全长cDNA，它们被鉴定为MAGE-1、MAGE-3、MAGE-12以及两个此前未描述过的MAGE基因，即MAGE-3b和MAGE-X2。对MAGE-3b和MAGE-X2基因编码区的DNA序列分析显示，它们与MAGE-1的同源性分别为83%和88%，而MAGE-3b与全长MAGE-3克隆的同源性为98%。MAGE-X2和MAGE-3b的预测氨基酸序列包含共有HLA-A1肽结合基序，这表明，与MAGE-1一样，它们可能编码肿瘤相关抗原。此外，直接结合研究表明，MAGE-3和MAGE-12基因编码的一个九聚体肽含有HLA-A2的聚集体表位。
