Nakatsuru S, Sudo K, Nakamura Y
Department of Biochemistry, Cancer Institute, Tokyo, Japan.
Biochem Biophys Res Commun. 1994 Jul 15;202(1):82-7. doi: 10.1006/bbrc.1994.1896.
We isolated a novel human cDNA, termed hCDC10, whose predicted product showed a high degree of homology to the CDC10 protein of Saccharomyces cerevisiae. This cDNA contained an open reading frame of 1254 nucleotides encoding 418 amino acids, which included a GTP-binding motif, GX4GKS--DX2G--KXD. The predicted peptide sequence also revealed partial amino-acid identity (40-50%) with Diff 6 in Drosophila and with H5 in mouse. Each of these sequence homologues, including Saccharomyces cerevisiae CDC10, contains the GTP-binding motif. Northern blot analyses indicated that the hCDC10 gene is expressed ubiquitously in normal tissues.
我们分离出一种新的人类cDNA，命名为hCDC10，其预测产物与酿酒酵母的CDC10蛋白具有高度同源性。该cDNA包含一个1254个核苷酸的开放阅读框，编码418个氨基酸，其中包括一个GTP结合基序，即GX4GKS--DX2G--KXD。预测的肽序列还显示与果蝇中的Diff 6和小鼠中的H5有部分氨基酸同一性（40-50%）。这些序列同源物中的每一个，包括酿酒酵母CDC10，都含有GTP结合基序。Northern印迹分析表明，hCDC10基因在正常组织中普遍表达。
