Levitt J D
Anesthesiology. 1975 Mar;42(3):267-74. doi: 10.1097/00000542-197503000-00006.
Synaptosomes, or nerve-ending particles, were isolated from the cerebral cortices of young rats by homogenization, differential centrifugation, and density-gradient centrifugation. The sodium-potassium-activated adenosine triphosphatase enzyme system [(Na+ plus K+)-ATPase] of these particles is believed to represent in vitro the sodium-potassium pump of the nerve terminal. Suspensions of synpatosomes were equilibrated with air containing various concentrations of halothane and enflurane, as determined by gas chromatography. Clinical concentrations of the anesthetics had no effect on (Na+ plus K+)-ATPase activity. Fourteen per cent halothane and 14.8 per cent enflurane in the gas phase resulted in 12 and 10 per cent inhibition, respectively, of (Na+ plus K+)-ATPase activity. These data confirm that interference with active cation transport by inhibition of neuronal (Na+ plus K+)-ATPase is not related to the mechanism of halothane or enflurane anesthesia. (Key words: Anesthetics, volatile, halothane; Anesthetics, volatile, enflurane; Metabolism, enzymes, ATPase; Nerve, synaptosomal ATPase; Theories of anesthesia, ATPase.).
通过匀浆、差速离心和密度梯度离心从幼鼠大脑皮层中分离出突触体或神经末梢颗粒。这些颗粒的钠钾激活三磷酸腺苷酶系统[（Na⁺+K⁺）-ATP酶]被认为在体外代表神经末梢的钠钾泵。通过气相色谱法测定，使突触体悬浮液与含有不同浓度氟烷和恩氟烷的空气达到平衡。麻醉剂的临床浓度对（Na⁺+K⁺）-ATP酶活性没有影响。气相中14%的氟烷和14.8%的恩氟烷分别导致（Na⁺+K⁺）-ATP酶活性受到12%和10%的抑制。这些数据证实，通过抑制神经元（Na⁺+K⁺）-ATP酶对活性阳离子转运的干扰与氟烷或恩氟烷麻醉机制无关。（关键词：麻醉剂，挥发性，氟烷；麻醉剂，挥发性，恩氟烷；代谢，酶，ATP酶；神经，突触体ATP酶；麻醉理论，ATP酶。）
