Verger J M, Gâté M, Piéchaud M, Chatelain R, Ramisse J, Blancou J
Ann Microbiol (Paris). 1975 Jan;126(1):57-74.
A Gram-negative organism isolated from a btich, in Madagascar, was examined by bacteriologic, immunologic and metabolic methods, in parallel with cultures representative of the Brucella species. The organism fits well into the genus Brucella on the basis of its growth, biochemical and antigenic characteristics and was found to have the metabolic pattern on L-asparagine (-), L-arginine (+) and DL-ornithine (+) that identifies and defines the species Brucella suis. It is of rough colonial morphology and electron microscopy showed a cell wall structure similar to that of other rough Brucella. By all the other recommended criteria for btotype identification it was found to be similar to Brucella suis biotype 5 best known as Brucella canis. In contrast to the strains of this biotype, it grows on basic fuchsin at 20 mug/ml and on safranine O at 200 mug/ml. These differences obtained with just one strain would not justify by now the proposal for a new biotype. We favor the designation Brucella suis biotype 5 proposed by Meyer, and the validity of Brucella canis (Carmichael and Bruner) as a separate species is discussed. It is the first strain of Brucella isolated in Madagascar.
从马达加斯加一名患者的伤口分离出的一株革兰氏阴性菌，采用细菌学、免疫学和代谢方法进行了检测，并与布鲁氏菌属的代表性培养物进行了对比。根据其生长、生化和抗原特性，该菌完全符合布鲁氏菌属的特征，并且发现其在L-天冬酰胺（-）、L-精氨酸（+）和DL-鸟氨酸（+）上具有代谢模式，这确定并定义了猪布鲁氏菌这一物种。它具有粗糙的菌落形态，电子显微镜显示其细胞壁结构与其他粗糙型布鲁氏菌相似。根据所有其他推荐的原型鉴定标准，发现它与最著名的犬布鲁氏菌（Brucella canis）的猪布鲁氏菌生物型5最为相似。与该生物型的菌株不同，它能在浓度为20微克/毫升的碱性品红和浓度为200微克/毫升的番红O上生长。仅从一个菌株获得的这些差异目前还不足以支持提出一个新的生物型。我们赞成迈耶提出的猪布鲁氏菌生物型5的命名，并讨论了犬布鲁氏菌（Carmichael和Bruner）作为一个独立物种的有效性。这是在马达加斯加分离出的第一株布鲁氏菌菌株。
