Ushakova N A, Samojlova R S, Popova I A, Presnova V N, Preobrazhenskaya M E
Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow.
Biochem Mol Biol Int. 1994 Mar;32(4):659-69.
Isoform patterns of six lysosomal glycosidases were studied in leukemic lymphoid cells phenotypically related to B and T cells at distinct stages of differentiation. In all types of cells, the activity of glycosidases under study was expressed in two major isoforms. No correlation was observed between isoform patterns and cell phenotypes. The beta-hexosaminidase isoform ratios for phenotypically related leukemic lymphoid cells but isolated from different sources (blood and spleen) differed. It was suggested that cell localization affects isoform expression. An anomalous alpha-mannosidase was detected in lymphoid cells from lymph nodes, while it was lacking in the phenotypically related blood lymphoid cells from the same patients. Isoform I of beta-hexosaminidase was recorded in lymphoid cells of patients with anemias.
在分化不同阶段与B细胞和T细胞表型相关的白血病淋巴细胞中，研究了六种溶酶体糖苷酶的同工型模式。在所有类型的细胞中，所研究的糖苷酶活性以两种主要同工型表达。未观察到同工型模式与细胞表型之间的相关性。表型相关但来自不同来源（血液和脾脏）的白血病淋巴细胞的β-己糖胺酶同工型比率不同。提示细胞定位影响同工型表达。在淋巴结的淋巴细胞中检测到一种异常的α-甘露糖苷酶，而在同一患者表型相关的血液淋巴细胞中则不存在。β-己糖胺酶同工型I在贫血患者的淋巴细胞中被记录到。
