Ushakova N A, Samojlova R S, Preobrazhenskaya M E
Institute of Biological and Medical Chemistry, Russian Academy of Medical Sciences, Moscow.
Biochem Int. 1992 Dec;28(6):1079-87.
The activity of acid alpha-mannosidase in phenotypically characterized lymphoid cells, isolated from peripheral blood, spleen and lymph nodes of patients with various lymphoproliferative disorders has been studied. Cells with different immunophenotypes were shown to have different alpha-mannosidase activity levels. The lowest alpha-mannosidase activity was observed in cells phenotypically corresponding to early B cells obtained from B-CLL patients. The highest activity was determined in cells with phenotypes of activated, CD11c-expressing B cells from B-NHL and HCL patients. There were considerable differences in alpha-mannosidase activity between peripheral blood and spleen lymphoid cells of B-NHL patients with spleen damage. The data obtained may be used in classification, primary diagnosis and staging of hematopoietic malignancies.
对从患有各种淋巴增殖性疾病患者的外周血、脾脏和淋巴结中分离出的、经表型鉴定的淋巴细胞中的酸性α-甘露糖苷酶活性进行了研究。结果显示，具有不同免疫表型的细胞具有不同水平的α-甘露糖苷酶活性。在表型上对应于从B-CLL患者获得的早期B细胞的细胞中观察到最低的α-甘露糖苷酶活性。在来自B-NHL和HCL患者的具有活化的、表达CD11c的B细胞表型的细胞中测定到最高活性。脾脏受损的B-NHL患者的外周血和脾脏淋巴细胞之间的α-甘露糖苷酶活性存在显著差异。所获得的数据可用于造血系统恶性肿瘤的分类、初步诊断和分期。
