Elofsson M, Broddefalk J, Ekberg T, Kihlberg J
Chemical Center, University of Lund, Sweden.
Carbohydr Res. 1994 May 20;258:123-33. doi: 10.1016/0008-6215(94)84080-6.
N alpha-Fmoc-serine pentafluorophenyl ester was glycosylated with perbenzoylated lactosyl bromide. The resulting product was coupled to a resin functionalized with 6-aminohexanoic acid and then N alpha-acylated to give a serine-based analogue of lactosylceramide. The water-soluble neoglycolipid was covalently linked to microtiter plates via its carboxyl group and was recognized by a lactose-binding lectin in an ELISA.
