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A sensitive and specific enzyme immunoassay for cyclomaltoheptaose and some derivatives.

作者信息

Créminon C, Pilard F, Grassi J, Perly B, Pradelles P

机构信息

CEA, DRIPP, Service de Pharmacologie et d'Immunologie, Centre d'Etudes de Saclay, Gif s/Yvette, France.

出版信息

Carbohydr Res. 1994 May 20;258:179-86. doi: 10.1016/0008-6215(94)84084-9.

DOI:10.1016/0008-6215(94)84084-9
PMID:8039174
Abstract

Antibodies were raised against cyclomaltoheptaose (beta-cyclodextrin), providing a highly sensitive enzyme immunoassay for beta-cyclodextrin and some derivatives with a detection limit of 120 pg/mL. Investigations of cross-reactivities with a wide variety of linear and cyclic maltooligosaccharides demonstrate that the antibodies are highly specific for cyclomaltoheptaose and a number of derivatives. The epitope is probably located on the secondary hydroxyl groups of the rim side. This enzyme immunoassay is shown to be suitable to detect cyclomaltoheptaose in urine and in plasma.

摘要

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