Department of Applied Biology, Kyoto Institute of Technology, Kyoto, Japan,
Cytotechnology. 2002 Nov;40(1-3):23-9. doi: 10.1023/A:1023997501517.
Cyclomaltoheptaose (beta-cyclodextrin, beta-CD) is a promising compound for application in various industrial fields because of its ability to entrap various compounds into its hydrophobic cavity. A monoclonal antibody (A7) to beta-CD was generated by using a conjugate of glucosaminylmaltosyl-beta-CD and bovine serum albumin as an antigen. The A7 monoclonal antibody was IgM/kappa and reacted with beta-CD with high specificity. The epitope recognized by the A7 monoclonal antibody seemed to be located on the secondary hydroxyl groups of the rim side of the beta-CD molecule. The dissociation constant of the complex of beta-CD and the immobilized A7 monoclonal antibody was determined to be 1.2 x 10(-4) M. A competitive ELISA using the A7 monoclonal antibody enabled determination of beta-CD and its derivatives with a detection limit of 0.05 muM. This immunoassay was useful to determine beta-CD in biological fluids such as human plasma and urine after appropriate pretreatment of the samples.
环麦芽七糖（β-环糊精，β-CD）是一种很有前途的化合物，由于其能够将各种化合物包埋到其疏水性空腔中，因此可应用于各个工业领域。通过使用葡糖胺基麦芽糖基-β-CD 与牛血清白蛋白的缀合物作为抗原，生成了针对β-CD 的单克隆抗体（A7）。A7 单克隆抗体为 IgM/κ，与β-CD 具有高度特异性反应。A7 单克隆抗体识别的表位似乎位于β-CD 分子边缘的次级羟基上。β-CD 与固定化 A7 单克隆抗体的复合物的解离常数为 1.2×10^(-4)M。使用 A7 单克隆抗体进行的竞争性 ELISA 能够检测β-CD 及其衍生物，检测限为 0.05μM。该免疫测定法在对样品进行适当预处理后，可用于测定人血浆和尿液等生物体液中的β-CD。
