Natsume M, Kamo Y, Hirayama M, Adachi T
Bio Science Laboratories, Saitama, Japan.
Carbohydr Res. 1994 May 20;258:187-97. doi: 10.1016/0008-6215(94)84085-7.
Lytic digestion of poly(mannuronate), poly(guluronate), and alginate with an alginate lyase from Alteromonas macleodii was used to prepare mixtures of unsaturated oligosaccharides. Four oligosaccharides isolated from the alginate lyase-lysate by anion-exchange chromatography on Q-Sepharose were found to be the major components of the root growth-promoting lysate. The oligosaccharides were analyzed by NMR and SIMS and identified as di- and tri-saccharides having O-(4-deoxy-L-erythro-hex-4-enopyranosyluronic acid)-1--> at the nonreducing terminus. The trisaccharides from the lysate were found to have root growth-promoting activity in a barley bioassay.
利用来自麦克劳德交替单胞菌的一种海藻酸裂合酶对聚甘露糖醛酸、聚古洛糖醛酸和海藻酸盐进行裂解消化，以制备不饱和低聚糖混合物。通过在Q-琼脂糖上进行阴离子交换色谱法从海藻酸裂合酶裂解物中分离出的四种低聚糖被发现是促进根系生长的裂解物的主要成分。通过核磁共振（NMR）和二次离子质谱（SIMS）对这些低聚糖进行分析，并鉴定为在非还原末端具有O-(4-脱氧-L-赤藓糖己-4-烯吡喃糖醛酸)-1→的二糖和三糖。在大麦生物测定中发现，裂解物中的三糖具有促进根系生长的活性。
