Hashino J, Fukuda Y, Oikawa S, Nakazato H, Nakanishi T
Suntory Institute for Biomedical Research, Osaka, Japan.
Clin Exp Metastasis. 1994 Jul;12(4):324-8. doi: 10.1007/BF01753839.
In order to examine a role of carcinoembryonic antigen (CEA) in metastasis, cDNA encoding CEA was introduced into a clone of human colorectal carcinoma SW1222 cells. Western blot analysis revealed that all transfectants express CEA of 180 kDa while the parent clone does not. In the transfectants, the level of CEA expression in clone 3 was higher than that of clone 1. Clone 3 formed aggregates rapidly after suspended by trypsinization while clone 1 did not. In experimental metastasis assay where tumor cells were injected intrasplenically, clone 3 exhibited a higher liver-metastatic activity than clone 1. Fab fragment of anti-CEA antibody significantly inhibited both the cell aggregation and the liver metastases caused by clone 3. These findings suggested that CEA expressed on the cell surface may play an important role in hepatic metastasis from colorectal carcinoma, possibly through its cell adhesion activity.
为了研究癌胚抗原（CEA）在转移中的作用，将编码CEA的cDNA导入人结肠直肠癌SW1222细胞的一个克隆中。蛋白质免疫印迹分析显示，所有转染子均表达180 kDa的CEA，而亲本克隆不表达。在转染子中，克隆3中CEA的表达水平高于克隆1。用胰蛋白酶消化悬浮后，克隆3迅速形成聚集体，而克隆1则不会。在将肿瘤细胞经脾内注射的实验性转移试验中，克隆3表现出比克隆1更高的肝转移活性。抗CEA抗体的Fab片段显著抑制了克隆3引起的细胞聚集和肝转移。这些发现表明，细胞表面表达的CEA可能在结直肠癌肝转移中起重要作用，可能是通过其细胞黏附活性。
