Kozlowski J M, Fidler I J, Campbell D, Xu Z L, Kaighn M E, Hart I R
Cancer Res. 1984 Aug;44(8):3522-9.
The metastatic behavior of seven human tumor cell lines grown in young (3- to 4-week-old) nude mice was studied. Two cell lines were derived from malignant melanomas, one from a colon carcinoma, two from prostate adenocarcinomas, and two from renal adenocarcinomas. Many of the cell lines produced metastases after i.v. injection (experimental metastasis) and after s.c. transplantation (spontaneous metastasis) into young nude mice. The incidence of metastasis seemed dependent primarily on the biological characteristics of the individual tumor cell line. However, the incidence of metastasis of some tumor cell lines could be increased by isolation and establishment of variant sublines from secondary tumor deposits, by prolonged systemic administration of 17 beta-estradiol to suppress natural killer cell activity, and/or by use of an advantageous site of tumor implantation. Intrasplenic injection of tumor cells allowed the most dramatic overall expression of metastatic capacity in these cell lines, resulting in frequent and large metastases to liver, lungs, and the mesenteric, omental, and mediastinal lymph nodes.
对在年轻（3至4周龄）裸鼠体内生长的七种人类肿瘤细胞系的转移行为进行了研究。其中两种细胞系源自恶性黑色素瘤，一种源自结肠癌，两种源自前列腺腺癌，还有两种源自肾腺癌。许多细胞系在静脉注射（实验性转移）以及皮下移植（自发性转移）到年轻裸鼠体内后都会产生转移。转移发生率似乎主要取决于各个肿瘤细胞系的生物学特性。然而，某些肿瘤细胞系的转移发生率可通过从继发性肿瘤沉积物中分离并建立变异亚系、长期全身性给予17β-雌二醇以抑制自然杀伤细胞活性和/或通过使用有利的肿瘤植入部位来提高。脾内注射肿瘤细胞能使这些细胞系的转移能力得到最显著的整体表现，导致频繁且大量地转移至肝脏、肺以及肠系膜、网膜和纵隔淋巴结。
