Lahbib-Mansais Y, Mellink C, Yerle M, Gellin J
Laboratoire de Génétique Cellulaire, Centre INRA Toulouse, Castanet-Tolosan, France.
Cytogenet Cell Genet. 1994;67(2):120-5. doi: 10.1159/000133811.
The nerve growth factor beta gene (NGFB) belongs to a conserved syntenic group on human chromosome 1 and mouse Chromosome 3. The objective of this study was the isolation of a part of the porcine NGFB gene and its use as a genetic and physical marker in the pig genome. On the basis of a nucleotide sequence comparison among different species, NGFB-specific primers were chosen to amplify the corresponding porcine sequence by PCR. A pig genomic DNA fragment of 763 bp was isolated, and its DNA sequence, containing the complete coding sequence of mature NGFB, was determined. It was demonstrated that pig NGFB is largely homologous with NGFB of other species (mouse, cattle, chicken) and especially with human NGFB; the isolated clone shows 91% nucleotide and 99% translated amino acid sequence identity to the human NGFB sequence. The porcine DNA clone allowed us to identify an RFLP marker for genetic mapping in pigs and was used to map the NGFB gene to pig chromosome region 4q1.6-->q2.3 by radioactive in situ hybridization. Previously, we had localized to the same chromosome another member of this syntenic group, the gene encoding alpha 1 Na+,K+ ATPase (ATP1A1). These results show that a portion of the homologous region between human chromosome 1 and mouse Chromosome 3 is also conserved on pig chromosome 4.
神经生长因子β基因（NGFB）属于人类1号染色体和小鼠3号染色体上的一个保守同线群。本研究的目的是分离猪NGFB基因的一部分，并将其用作猪基因组中的遗传和物理标记。基于不同物种间的核苷酸序列比较，选择NGFB特异性引物通过PCR扩增相应的猪序列。分离出一个763 bp的猪基因组DNA片段，并测定了其DNA序列，该序列包含成熟NGFB的完整编码序列。结果表明，猪NGFB与其他物种（小鼠、牛、鸡）的NGFB在很大程度上具有同源性，尤其与人类NGFB同源；分离的克隆与人类NGFB序列的核苷酸同一性为91%，翻译后的氨基酸序列同一性为99%。该猪DNA克隆使我们能够鉴定出用于猪遗传图谱绘制的RFLP标记，并通过放射性原位杂交将NGFB基因定位到猪染色体区域4q1.6→q2.3。此前，我们已将该同线群的另一个成员，即编码α1 Na +,K + ATP酶（ATP1A1）的基因定位到同一染色体上。这些结果表明，人类1号染色体和小鼠3号染色体之间的部分同源区域在猪4号染色体上也得到了保守。
