基于16S rRNA序列分析的嗜酸氢杆菌的系统发育位置。
The phylogenetic position of Hydrogenobacter acidophilus based on 16S rRNA sequence analysis.
作者信息
Shima S, Yanagi M, Saiki H
机构信息
Department of Biotechnology, Central Research Institute of Electric Power Industry, Chiba, Japan.
出版信息
FEMS Microbiol Lett. 1994 Jun 1;119(1-2):119-22. doi: 10.1111/j.1574-6968.1994.tb06877.x.
Hydrogenobacter acidophilus strain 3H-1 is a thermoacidophilic, obligately chemolithoautotrophic, hydrogen-oxidizer isolated from a Japanese solfataric field. Strain 3H-1 requires elemental sulfur for growth. We used PCR to amplify the 16S rRNA gene of strain 3H-1, and sequenced the amplification product directly. Phylogenetic analyses show strain 3H-1 is closely related to Aquifex pyrophilus and may be located in the deepest branch within the eubacterial phylogenetic tree. Sulfur-dependency of the ancestral eubacterium is also discussed.
嗜酸氢杆菌3H-1菌株是从日本一处硫质喷气孔场分离得到的嗜热嗜酸、专性化能无机自养型氢氧化菌。3H-1菌株生长需要单质硫。我们使用聚合酶链反应（PCR）扩增3H-1菌株的16S核糖体RNA（rRNA）基因，并直接对扩增产物进行测序。系统发育分析表明，3H-1菌株与嗜热栖热菌密切相关，可能位于真细菌系统发育树的最深处分支。文中还讨论了原始真细菌对硫的依赖性。
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