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枯草芽孢杆菌孢子形成过程中的新转移核糖核酸种类

New transfer ribonucleic acid species during sporulation of Bacillus subtilis.

作者信息

Jeng Y H, Doi R H

出版信息

J Bacteriol. 1975 Mar;121(3):950-8. doi: 10.1128/jb.121.3.950-958.1975.

Abstract

The transfer ribonucleic acid (tRNA) populations from log-phase cells, sporulating cells (stage III), and dormant spores were compared by tRNA-deoxyribonucleic acid hybridization techniques. New tRNA species not found in log-phase cells were observed in stage III cells. Some of the tRNA made during sporulation were also present in dormant spores. Although the role and function of these new tRNA species cannot be ascribed directly to the sporulation process, their presence indicates that new tRNA genes can be transcribed during sporulation and suggests that translational control may be exerted during sporulation by tRNA.

摘要

通过转运核糖核酸-脱氧核糖核酸杂交技术,对对数期细胞、芽孢形成细胞(III期)和休眠孢子中的转运核糖核酸(tRNA)群体进行了比较。在III期细胞中观察到了对数期细胞中未发现的新tRNA种类。芽孢形成过程中产生的一些tRNA也存在于休眠孢子中。尽管这些新tRNA种类的作用和功能不能直接归因于芽孢形成过程,但它们的存在表明在芽孢形成过程中可以转录新的tRNA基因,这表明在芽孢形成过程中可能通过tRNA进行翻译控制。

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1
ISOLATION AND PURIFICATION OF UNDEGRADED RIBONUCLEIC ACIDS.未降解核糖核酸的分离与纯化
Biochim Biophys Acta. 1964 May 18;87:9-16. doi: 10.1016/0926-6550(64)90041-6.
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Transfer RNA and cell differentiation.转运RNA与细胞分化
Prog Nucleic Acid Res Mol Biol. 1970;10:23-55. doi: 10.1016/s0079-6603(08)60560-7.
6
Nucleic acid reassociation in formamide.核酸在甲酰胺中的重缔合
Biochemistry. 1969 Aug;8(8):3289-95. doi: 10.1021/bi00836a024.

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