两种用于电子显微镜检查的铁蛋白 - 蛋白质缀合物的改进制备方法。
Two improved methods for preparing ferritin-protein conjugates for electron microscopy.
作者信息
Kishida Y, Olsen B R, Berg R A, Prockop D J
出版信息
J Cell Biol. 1975 Feb;64(2):331-9. doi: 10.1083/jcb.64.2.331.
Abstract
Two improved procedures were developed for activating ferritin so that the ferritin could be covalently linked to antibodies. One procedure involved use of a water-soluble carbodiimide and N-hydroxysuccinimide to prepare ferritin-containing activated esters. The other involved activation of the ferritin with excess glutaraldehyde. The ferritin-antibody conjugates prepared with the two procedures were shown to have a number of properties which made them suitable for locating antigenic components in cells.
摘要
开发了两种改进的方法来激活铁蛋白，以便铁蛋白能够与抗体共价连接。一种方法是使用水溶性碳二亚胺和N-羟基琥珀酰亚胺来制备含铁蛋白的活化酯。另一种方法是用过量的戊二醛激活铁蛋白。用这两种方法制备的铁蛋白-抗体缀合物显示出许多特性，使其适合于定位细胞中的抗原成分。
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本文引用的文献
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