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由痘苗病毒表达的恶性疟原虫富含谷氨酸蛋白的免疫原性。

Immunogenicity of the Plasmodium falciparum glutamate-rich protein expressed by vaccinia virus.

作者信息

Theisen M, Cox G, Høgh B, Jepsen S, Vuust J

机构信息

Department of Infection-Immunology, Statens Seruminstitut, Copenhagen, Denmark.

出版信息

Infect Immun. 1994 Aug;62(8):3270-5. doi: 10.1128/iai.62.8.3270-3275.1994.

DOI:10.1128/iai.62.8.3270-3275.1994
PMID:8039897
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC302955/
Abstract

The glurp gene of Plasmodium falciparum F32 has been inserted into a vaccinia virus, and the recombinant virus was designated VVG4. Expression of glurp in VVG4-infected Vero cells was analyzed by immunoprecipitation and revealed a primary GLURP product of approximately 220,000 Da; GLURP was detected both intracellularly and in culture supernatants. To study the immunogenicity of vaccinia virus-expressed GLURP, mice were immunized with VVG4 and serum samples were analyzed for antibody reactivity with three polypeptides, covering almost the entire GLURP molecule; these three polypeptides were produced in recombinant form in Escherichia coli. The immune response was primarily directed against a carboxy-terminal repeat region. The mouse anti-GLURP serum recognized authentic GLURP by immunoprecipitation analysis from P. falciparum grown in vitro. These results demonstrate that vaccinia virus-expressed glurp product can induce a humoral immune response against GLURP derived from blood-stage parasites.

摘要

恶性疟原虫F32的glurp基因已被插入痘苗病毒中，这种重组病毒被命名为VVG4。通过免疫沉淀分析了glurp在VVG4感染的Vero细胞中的表达情况，结果显示主要的GLURP产物约为220,000道尔顿；在细胞内和培养上清液中均检测到了GLURP。为了研究痘苗病毒表达的GLURP的免疫原性，用VVG4免疫小鼠，并分析血清样本与三种多肽的抗体反应性，这三种多肽几乎覆盖了整个GLURP分子；这三种多肽以重组形式在大肠杆菌中产生。免疫反应主要针对羧基末端重复区域。通过对体外培养的恶性疟原虫进行免疫沉淀分析，小鼠抗GLURP血清可识别天然的GLURP。这些结果表明，痘苗病毒表达的glurp产物可诱导针对来自血液期寄生虫的GLURP的体液免疫反应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dda9/302955/208c07ac7ef7/iai00008-0242-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dda9/302955/692acb0e4d9e/iai00008-0240-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dda9/302955/2873e131b5fe/iai00008-0241-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dda9/302955/bd119fdb9a59/iai00008-0241-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dda9/302955/208c07ac7ef7/iai00008-0242-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dda9/302955/692acb0e4d9e/iai00008-0240-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dda9/302955/2873e131b5fe/iai00008-0241-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dda9/302955/bd119fdb9a59/iai00008-0241-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dda9/302955/208c07ac7ef7/iai00008-0242-a.jpg

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